| CAS Number | 134678-17-4 |
|---|---|
| Molecular Formula | C8H11N3O3S |
| Molecular Weight | 229.250 |
| InChI Key | JTEGQNOMFQHVDC-RQJHMYQMSA-N |
| LogP | -0.930 |
| Synonyms |
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Applications:
UV-Vis Spectrum of Lamivudine
July 11, 2025
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC-MS Method for Analysis of Lamivudine on Primesep 100 Column
February 10, 2025
HPLC Method for Analysis of Lamivudine on Primesep 100 by SIELC Technologies
Lamivudine is an antiviral medication used primarily to treat infections caused by the human immunodeficiency virus (HIV) and hepatitis B virus (HBV). It belongs to a class of drugs known as nucleoside reverse transcriptase inhibitors (NRTIs).
Uses:
- HIV Treatment – Lamivudine is commonly combined with other antiretroviral drugs to manage HIV infection and slow disease progression.
- Hepatitis B (HBV) Treatment – It helps suppress HBV replication and reduces liver damage.
Lamivudine can be retained, and analyzed using a Primesep 100 mix mode stationary phase column. The analysis utilizes a isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and Ammonium Formate pH 3.0 – 20 mM as a buffer. Detection is carried out using UV, LC-MS.
| Column | Primesep 100, 2.1 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 60/40% |
| Buffer | Ammonium Formate pH 3.0 – 20 mM |
| Flow Rate | 0.2 ml/min |
| Detection | UV at 277 nm; ESI-SIM: [M+H]⁺ 230, 271 |
| Class of Compounds | Drug |
| Analyzing Compounds | Lamivudine |
Application Column
Primesep 100
Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
LC MS Detection
Separation of Lamivudine on Newcrom R1 HPLC column
February 19, 2018
Lamivudine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options
