Lamivudine

Lamivudine structural formula

CAS Number134678-17-4
Molecular FormulaC8H11N3O3S
Molecular Weight229.250
InChI KeyJTEGQNOMFQHVDC-RQJHMYQMSA-N
LogP-0.930
Synonyms
  • Lamivudine
  • 4-Amino-1-[(2S,5R)-2-(hydroxymethyl)-1,3-oxathiolan-5-yl]pyrimidin-2(1H)-one
  • 2(1H)-Pyrimidinone, 4-amino-1-[(2S,5R)-2-(hydroxymethyl)-1,3-oxathiolan-5-yl]-
  • 134678-17-4
  • 3TC
  • 2(1H)-Pyrimidinone, 4-amino-1-[(2R,5S)-2-(hydroxymethyl)-1,3-oxathiolan-5-yl]-
  • (-)-BCH-189
  • Heptovir
  • (-)-2'-Deoxy-3'-thiacytidine
  • 4-Amino-1-((2R,5S)-2-(hydroxymethyl)-1,3-oxathiolan-5-yl)-2(1H)-pyrimidinone
  • BCH 189, (-)-
  • 3'-Thia-2',3'-dideoxycytidine
  • (-)-BCH 189
  • Epivir-HBV
  • beta-L-2',3'-Dideoxy-3'-thiacytidine
  • beta-L-3'-Thia-2',3'-dideoxycytidine
  • Hepitec
  • UNII-2T8Q726O95
  • 2',3'-Dideoxy-3'-thiacytidine

Applications:

HPLC-MS Method for Analysis of Lamivudine on Primesep 100 Column

February 10, 2025

HPLC Method for Analysis of Lamivudine on Primesep 100 by SIELC Technologies

HPLC Method for Analysis of Lamivudine on Primesep 100 Column by SIELC Technologies

Lamivudine is an antiviral medication used primarily to treat infections caused by the human immunodeficiency virus (HIV) and hepatitis B virus (HBV). It belongs to a class of drugs known as nucleoside reverse transcriptase inhibitors (NRTIs).

Uses:

  1. HIV Treatment – Lamivudine is commonly combined with other antiretroviral drugs to manage HIV infection and slow disease progression.
  2. Hepatitis B (HBV) Treatment – It helps suppress HBV replication and reduces liver damage.

Lamivudine can be retained, and analyzed using a Primesep 100 mix mode stationary phase column. The analysis utilizes a isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and Ammonium Formate pH 3.0 – 20 mM as a buffer. Detection is carried out using UV, LC-MS.

ColumnPrimesep 100, 2.1 x 100 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O – 60/40%
BufferAmmonium Formate pH 3.0 – 20 mM
Flow Rate0.2 ml/min
DetectionUV at 277 nm; ESI-SIM: [M+H]⁺ 230, 271
Class of CompoundsDrug
Analyzing CompoundsLamivudine

Application Column

Primesep 100

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Lamivudine
Application Detection:
UV Detection
LC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Lamivudine on Newcrom R1 HPLC column

February 19, 2018
Separation of Lamivudine on Newcrom C18 HPLC column

Lamivudine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Lamivudine
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.