HPLC Method for Analysis of Lactulose on Primesep S2 Column

HPLC Method for Lactulose on Primesep S2 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Lactulose

Lactulose is an synthetic disaccharide with the molecular formula C12H22O11.

Properties:
Appearance: Typically a white, odorless crystalline powder with a sweet taste.

Molecular weight: ~342.30 g/mol

Solubility: Soluble in water.

Uses: Used for treating constipation and hepatic encephalopathy (HE). 

Lactulose can be retained and analyzed using the Primesep S2 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and formic acid. Detection is performed using ELSD.

ColumnPrimesep S2, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 80%
BufferFormic Acid – 0.2%
Flow Rate1.0 ml/min
DetectionELSD
LOD7.643 ppm

Class of CompoundsSynthetic Disaccharide
Analyzing CompoundsLactulose

Application Column

Primesep S2

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Lactulose
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.