HPLC Method for Analysis of Isonicotinic Acid on Primesep 100 Column on Alltesta™

HPLC Method for Isonicotinic Acid on Primesep 100 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Isonicotinic Acid

Isonicotinic Acid is an nucleoside with the C6H5NO2 molecular formula. It is a derivative of pyridine, making it a pyridinemonocarboxylic acid. It acts primarily as a metabolite in humans and algae.

Isonicotinic Acid can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 30%
BufferH2SO4 – 0.1%
Flow Rate1.0 ml/min
DetectionUV 275 nm

Class of CompoundsAcid
Analyzing CompoundsIsonicotinic Acid

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Isonicotinic Acid
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.