HPLC Method for Analysis of Hydroquinone on Primesep 100 Column on Alltesta™

HPLC Method for Hydroquinone on Primesep 100 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Hydroquinone

Hydroquinone is an aromatic derivative of benzene with the chemical formula C6H6O2. It is often used in skin whitening, although it has been banned by the United States Food and Drug Administration for over-the-counter use due to being a potential carcinogen. It can cause a variety of disease including but not limited to ochronosis. thyroid follicular cell hyperplasias, mononuclear cell leukemia, and adenomas. Agencies across the world encourage research into other agents to treat hyperpigmentation. You can find detailed UV spectra of Hydroquinone and information about its various lambda maxima by visiting the following link.

Hydroquinone can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 70%
BufferSulfuric Acid
Flow Rate1.0 mL/min
DetectionUV 275 nm
Class of CompoundsPhenols
Analyzing CompoundsHydroquinone

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Hydroquinone
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.