HPLC Method for Analysis of Formic Acid and Acetic Acid on Chromni Column

HPLC Method for Analysis of Formic acid, Acetic Acid on Chromni Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Formic acid, Acetic Acid.

Formic Acid is the simplest carboxylic acid with the chemical formula CH2O2. It is naturally found in insects, weeds, fruits, and vegetables. It is used by insects as a method of self-defense. In agriculture, it is used as a preservative and antibacterial agent. In chromatography, it is used as a volatile pH modifier. It is used significantly in the tanning of leather, dyeing and finishing of textiles, and production of rubber. You can find detailed UV spectra of Formic Acid and information about its various lambda maxima by visiting the following link.

Acetic Acid is a simple carboxylic acid with the formula CH3COOH. It’s an important chemical reagent and industrial chemical, primarily used in the production of cellulose acetate for photographic film and polyvinyl acetate for wood glue, as well as synthetic fibers and fabrics. It gives vinegar its sour taste and pungent smell. It is well known for being the active ingredient in vinegar, leading to the belief that it is the earliest mass produced acid, dating back to 3BC.

Formic acid, Acetic Acid can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV.

Condition

ColumnChromni, 4.6 x 150 mm, 3 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O
BufferSulfuric Acid
Flow Rate1.0 ml/min
DetectionUV 190 nm

Description

Class of CompoundsAcids
Analyzing CompoundsFormic acid, Acetic Acid

Application Column

Chromni

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Acetic Acid
Formic acid
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.