HPLC Method for Analysis of Fenvalerate on Newcrom B Column on Alltesta™

HPLC Method for Fenvalerate on Newcrom B by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Fenvalerate 

Fenvalerate is synthetic pyrethroid insecticide with the chemical formula C25H22ClNO3. It is used against a wide range of pests, partially due to it’s moderate mammalian toxicity following short-term and acute exposure. It is the most toxic to bees and fish.  Upon contact, it may irritate skin and eyes.

You can find detailed UV spectra of Fenvalerate and information about its various lambda maxima by visiting the following link.

Fenvalerate can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with ammonium formate as a buffer. Detection is performed using UV.

ColumnNewcrom B, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 70%
BufferAmmonium Formate
Flow Rate1.0 mL/min
DetectionUV 275 nm
Class of CompoundsInsecticide
Analyzing CompoundsFenvalerate

Application Column

Newcrom B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Fenvalerate
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.