HPLC Method for Analysis of Deoxyuridine on Chromite™ Column on Alltesta™

HPLC Method for Deoxyuridine on Chromni™ by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Deoxyuridine

Deoxyuridine (dU) is an nucleoside with the C9H12N2O5 molecular formula. While in living organisms it exists primarily to occasionally become a part of DNA, it’s variants, Idoxuridine and Trifluoride, work as antiviral drugs. If consumed, Deoxyuridine can be toxic.

Deoxyuridine can be retained and analyzed using the Chromni™ stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.

ColumnChromni™, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 90%
BufferAmFm pH 3.0 – 30 mM
Flow Rate1.0 ml/min
DetectionUV 275 nm

Class of CompoundsNucleotide Base
Analyzing CompoundsDeoxyuridine

Application Column

Chromni™

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Deoxyuridine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.