HPLC Method for Analysis of Chromium (III) Ion on Chromni Column

HPLC Method for Analysis of Chromium(III) on Chromni Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Chromium(III).

Chromium(III) is a nutrient with the chemical formula Cr3+. It is believed to be essential for human metabolism of insulin, sugar, and lipids. Though, the notion is currently contested. It can be found naturally in rocks, soil, plants, as well as various animal products.

Chromium(III) can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an isocraticmethod with a simple mobile phase consisting of water and acetonitrile (MeCN) and TFA. Detection is performed using ELSD.

Condition

ColumnChromni, 4.6 x 150 mm, 3 µm, 100 A, surface coated
Mobile PhaseMeCN/H2O – 80/10%
BufferTFA – 0.2%
Flow Rate1.0 ml/min
DetectionELSD
Limit Of Detection*3 ppm
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Description

Class of CompoundsNutrient
Analyzing CompoundsChromium(III)

Application Column

Chromni

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: surface coated

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Application Analytes:
Chromium(III)
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.