Cr+3
| Molecular Formula | Cr+3 |
|---|---|
| Molecular Weight | 51.996 |
| InChI Key | BFGKITSFLPAWGI-UHFFFAOYSA-N |
| Synonyms |
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Applications:
HPLC Method for Analysis of Chromium (III) Ion on Chromni Column
August 25, 2026
HPLC Method for Analysis of Chromium(III) on Chromni Column by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Chromium(III).
Chromium(III) is a nutrient with the chemical formula Cr3+. It is believed to be essential for human metabolism of insulin, sugar, and lipids. Though, the notion is currently contested. It can be found naturally in rocks, soil, plants, as well as various animal products.
Chromium(III) can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an isocraticmethod with a simple mobile phase consisting of water and acetonitrile (MeCN) and TFA. Detection is performed using ELSD.
Condition
| Column | Chromni, 4.6 x 150 mm, 3 µm, 100 A, surface coated |
| Mobile Phase | MeCN/H2O – 80/10% |
| Buffer | TFA – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Limit Of Detection* | 3 ppm |
Description
| Class of Compounds | Nutrient |
| Analyzing Compounds | Chromium(III) |
Application Column
Chromni
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: surface coated
HPLC Method for Analysis of Chromium(III) on BIST B+ Column
February 6, 2023
HPLC Method for Analysis of Chromium(III) on BIST B+ by SIELC Technologies
Chromium(III) is a nutrient with the chemical formula Cr3+. It is believed to be essential for human metabolism of insulin, sugar, and lipids. Though, the notion is currently contested. It can be found naturally in rocks, soil, plants, as well as various animal products.
Using SIELC’s newly introduced BIST™ method, Chromium(III) can be retained on a positively-charged anion-exchange BIST B+ column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively charged trivalent Chromium ion to the positively charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Chromium(III) can be separated, retained, and UV detected at 200 nm.
Condition
| Column | BIST B+, 4.6 x 50 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | Gradient MeCN – 60-20%, 10 min |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 nm |
| Peak Retention Time | 5.71 min |
| LOD | 0.4 ppm |
Description
| Class of Compounds | Ion |
| Analyzing Compounds | Chromium(III) |
Application Column
BIST B+
Column Diameter: 4.6 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
