HPLC Method for Analysis of Benzisothiazolinone (BIT) on Newcrom R1 Column

HPLC Method for Benzisothiazolinone (BIT), 1,2-benzisothiazol-3(2H)-one on Newcrom R1 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Benzisothiazolinone (BIT), 1,2-benzisothiazol-3(2H)-one.

Benzisothiazolinone, also written as BIT, is a microbicide and fungicide with the chemical formula C7H5NOS. It is used primarily as a preservative in a large variety of industries, from paints, to caulks, to cleaning products, to agriculture, and more. It is banned from being used in cosmetics in the EU.  You can find detailed UV spectra of Benzisothiazolinone (BIT), 1,2-benzisothiazol-3(2H)-one and information about its various lambda maxima by visiting the following link.

Benzisothiazolinone (BIT), 1,2-benzisothiazol-3(2H)-one can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a trifluoracetic acid buffer. Detection is performed using UV.

Condition
Column Newcrom R1, 3.2 x 50 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN – 10%
Buffer TFA- 0.1%
Flow Rate 0.5 ml/min
Detection UV 275nm

Description
Class of Compounds Acid, Hydrophilic
Analyzing Compounds Benzisothiazolinone (BIT), 1,2-benzisothiazol-3(2H)-one

Application Column

Newcrom R1

Column Diameter: 3.2 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Benzisothiazolinone (BIT), 1,2-benzisothiazol-3(2H)-one
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.