HPLC Method for B-Estradiol on Primesep 200 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of B-Estradiol
B-Estradiol is an estrogen steroid hormone. It is the major estrogen secreted by the premenopausal ovary. It is synthesized from testosterone primarily in the ovarian granulosa cells and placenta. B-estradiol is used as a medication, for instance in menopausal hormone therapy. Primesep 200, a reverse phase column, contains embedded acidic ionizable groups and can retain B-Estradiol. The method is UV compatible and can be used as a general approach for analyzing similar compounds.
| Column | Primesep 200, 3.2 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | Gradient MeCN – 5-50%, 5 min, 4 min hold |
| Buffer | Gradient H2SO4 – 0.05-0.3 %, 5 min, 4 min hold |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds |
Drug, Hormone, Hydrophobic |
| Analyzing Compounds | B-Estradiol |
Application Column
Primesep 200
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
B-EstradiolApplication Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
