HPLC Method for Sodium ascorbyl phosphate on Newcrom BH by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Sodium ascorbyl phosphate.
Sodium Ascorbyl Phosphate is a popular, more mild, form of Vitamin C with the chemical formula C6H6Na3O9P. It is found in a variety of dermatology products and used for its antioxidant properties.
Sodium ascorbyl phosphate can be retained and analyzed on a mixed-mode Newcrom BH column with a mobile phase consisting of water, Acetonitrile (MeCN), and either Sulfuric acid (H2SO4) or Phosphoric acid (H3PO4) as the ionic modifier. This analytical method can be UV detected at 238 nm with high resolution and peak symmetry.
| Column | Newcrom BH, 3.2 x 100 mm, 3 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | H2SO4 or H3PO4 |
| Flow Rate | 0.5 ml/min |
| Detection | UV 238 nm |
| LOD | 0.05 ppm |
| Class of Compounds | Acid, Hydrophilic |
| Analyzing Compounds | Sodium ascorbyl phosphate |
Application Column
Newcrom BH
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
Sodium ascorbyl phosphateApplication Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
