| Molecular Formula | C6H6Na3O9P |
|---|---|
| Molecular Weight | 322.05 |
| InChI Key | YRWWOAFMPXPHEJ-OFBPEYICSA-K |
| Synonyms |
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Applications:
Uv-Vis Spectrum of Sodium Ascorbyl Phosphate
March 9, 2026
Access the UV-Vis Spectrum SIELC Library

If you are looking for optimized HPLC method to analyze Sodium ascorbyl phosphate check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Method for Analysis of Ascorbyl Phosphate on Newcrom BH Column
August 2, 2022
HPLC Method for Sodium ascorbyl phosphate on Newcrom BH by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Sodium ascorbyl phosphate.
Sodium Ascorbyl Phosphate is a popular, more mild, form of Vitamin C with the chemical formula C6H6Na3O9P. It is found in a variety of dermatology products and used for its antioxidant properties.
Sodium ascorbyl phosphate can be retained and analyzed on a mixed-mode Newcrom BH column with a mobile phase consisting of water, Acetonitrile (MeCN), and either Sulfuric acid (H2SO4) or Phosphoric acid (H3PO4) as the ionic modifier. This analytical method can be UV detected at 238 nm with high resolution and peak symmetry.
| Column | Newcrom BH, 3.2 x 100 mm, 3 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | H2SO4 or H3PO4 |
| Flow Rate | 0.5 ml/min |
| Detection | UV 238 nm |
| LOD | 0.05 ppm |
| Class of Compounds | Acid, Hydrophilic |
| Analyzing Compounds | Sodium ascorbyl phosphate |
Application Column
Newcrom BH
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended


