Molecular Formula | C6H6Na3O9P |
---|---|
Molecular Weight | 322.05 |
InChI Key | YRWWOAFMPXPHEJ-OFBPEYICSA-K |
Synonyms |
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Applications:
HPLC Method for Analysis of Ascorbyl Phosphate on Newcrom BH Column
August 2, 2022
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ascorbyl Phosphate
Sodium Ascorbyl Phosphate is a popular, more mild form of Vitamin C found in a variety of dermatology products and used for its antioxidant properties. Ascorbyl Phosphate can be retained and analyzed on a mixed-mode Newcrom BH column with a mobile phase consisting of water, Acetonitrile (MeCN), and either Sulfuric acid (H2SO4) or Phosphoric acid (H3PO4) as the ionic modifier. This analytical method can be UV detected at 238 nm with high resolution and peak symmetry.
Column | Newcrom BH, 3.2×100 mm, 100A |
Mobile Phase | MeCN/H2O – 10/90% |
Buffer | H2SO4 or H3PO4 |
Flow Rate | 0.5 ml/min |
Detection | UV 238 nm |
LOD | 0.05 ppm |
Class of Compounds | Acid, Hydrophilic |
Analyzing Compounds | Ascorbyl phosphate |
Application Column
Newcrom BH
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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