HPLC Method for Analysis of Ascorbyl Phosphate on Newcrom BH Column

Separation type: Liquid Chromatography Mixed-mode

 

High Performance Liquid Chromatography (HPLC) Method for Analysis of Ascorbyl Phosphate

Sodium Ascorbyl Phosphate is a popular, more mild form of Vitamin C found in a variety of dermatology products and used for its antioxidant properties. Ascorbyl Phosphate can be retained and analyzed on a mixed-mode Newcrom BH column with a mobile phase consisting of water, Acetonitrile (MeCN), and either Sulfuric acid (H2SO4) or Phosphoric acid (H3PO4) as the ionic modifier. This analytical method can be UV detected at 238 nm with high resolution and peak symmetry.

 

 

 

Condition
Column Newcrom BH, 3.2×100 mm,  100A
Mobile Phase MeCN/H2O – 10/90%
Buffer H2SO4 or H3PO4
Flow Rate 0.5  ml/min
Detection UV 238 nm
LOD  0.05 ppm

Description
Class of Compounds Acid, Hydrophilic
Analyzing Compounds Ascorbyl phosphate

 

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Sodium ascorbyl phosphate
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.