HPLC Method for Analysis of Amoxicillin on Primesep 100 Column with Alltesta™

HPLC Method for Amoxicillin on Primesep 100 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Amoxicillin

Amoxicillin is a prescription antibiotic with the molecular formula C16H19N3O5S. It is typically used to treat bacterial infections and is taken orally.

Amoxicillin can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.

ColumnPrimesep 100, 3.2 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 50%
BufferSulfuric Acid
Flow Rate0.5 ml/min
DetectionUV 275 nm

Class of CompoundsAntibiotic
Analyzing CompoundsAmoxicillin

Application Column

Primesep 100

Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Amoxicillin
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.