HPLC Method for Analysis of Abaloparatide Using a BIST B+ Column

HPLC Method for Abaloparatide on BIST B+ by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Abaloparatide

Abaloparatide is a 34-amino acid parathyroid hormone-related protein (PTHrP) analogue with the molecular formula C174H299N56O49. It is used to treat osteoporosis in postmenopausal women who are intolerant to other available osteoporosis therapy. It is also used to increase bone density in men with osteoporosis.

Abaloparatide can be retained and analyzed using the BIST B+ stationary phase column. The analysis utilizes an gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV.

ColumnBIST B+, 4.6 x 50 mm, 5 µm, 300 A, dual ended
Mobile PhaseMeCN – 70-40%
BufferSulfuric Acid
Flow Rate1.0 ml/min
DetectionUV 200
LOD* UV180 ppb
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of CompoundsProtein Analogue
Analyzing CompoundsAbaloparatide

Application Column

BIST B+

Column Diameter: 4.6 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 300 A
Column options: dual ended

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Application Analytes:
Abaloparatide
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.