HPLC Method for Analysis of Hymexazol (3-Hydroxy-5-methylisoxazole) on Primesep B Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Hymexazol on Primesep B by SIELC Technologies

HPLC Method for Analysis of 3-Hydroxy-5-methylisoxazole on Primesep B Column
HPLC Method for Analysis of Hymexazol on Primesep B Column by SIELC Technologies

Hymexazol is a fungicide with the chemical formula C4H5NO2. It is a part of the class of isoxazoles which carry hydroxy and methyl substituents at positions 3 and 5 respectively. It is used primarily as a fungicide to control diseases in rice, sugarbeet, ornamentals, vegetables, and more. It works through inhibiting the fungal RNA and DNA synthesis. You can find detailed UV spectra of Hymexazol and information about its various lambda maxima by visiting the following link.

Hymexazol can be retained and analyzed using a Primesep B mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV at 222 nm

ColumnPrimesep B, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 10/90%
BufferH2SO4 – 0.05%
Flow Rate1.0 ml/min
DetectionUV 220 nm
Samples1.0 mg/ml in MeCN/H2O – 50/50%
Injection volume1 µl
LOD*7 ppb
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
Class of Compounds
Isoxazoles
Analyzing CompoundsHymexazol

Application Column

Primesep B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Hymexazol
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.