HPLC Method for Analysis of Hymexazol (3-Hydroxy-5-methylisoxazole) on Primesep B Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Hymexazol on Primesep B by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

HPLC Method for Analysis of 3-Hydroxy-5-methylisoxazole on Primesep B Column
HPLC Method for Analysis of Hymexazol on Primesep B Column by SIELC Technologies


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Hymexazol

3-Hydroxy-5-methylisoxazole can be retained and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV at 222 nm

ColumnPrimesep B, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O – 10/90%
BufferH2SO4 – 0.05%
Flow Rate1.0 ml/min
DetectionUV 220 nm
Samples1.0 mg/ml in MeCN/H2O – 50/50%
Injection volume1 µl
LOD*7 ppb
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Isoxazoles
Analyzing CompoundsHymexazol

Application Column

Primesep B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Hymexazol
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.