Hymexazol

Hymexazol structural formula

CAS Number10004-44-1
Molecular FormulaC4H5NO2
Molecular Weight99.089
InChI KeyKGVPNLBXJKTABS-UHFFFAOYSA-N
LogP0.46
Synonyms
  • Hymexazol
  • 5-Methyl-1,2-oxazol-3(2H)-one
  • 3(2H)-Isoxazolone, 5-methyl-
  • 10004-44-1
  • 5-Methyl-isoxazol-3-ol
  • EINECS 233-000-6
  • 3-Isoxazolol, 5-methyl-
  • Itachigarden
  • Tachigaren
  • UNII-20T2M875LO
  • 3-Hydroxy-5-methylisoxazole
  • 5-Methyl-3(2H)-isoxazolone
  • 5-Methyl-3-hydroxyisoxazole
  • 5-Methylisoxazol-3-ol
  • F 319
  • F-319
  • Hydroxyisoxazole
  • Hymexazole
  • NSC 217971
  • SF-6505
  • 128907-24-4
  • 29412-88-2
  • 52229-82-0

Applications:

HPLC Method for Analysis of Hymexazol (3-Hydroxy-5-methylisoxazole) on Primesep B Column

February 8, 2024

High Performance Liquid Chromatography (HPLC) Method for Analysis of Hymexazol on Primesep B by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

HPLC Method for Analysis of 3-Hydroxy-5-methylisoxazole on Primesep B Column
HPLC Method for Analysis of Hymexazol on Primesep B Column by SIELC Technologies


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Hymexazol

3-Hydroxy-5-methylisoxazole can be retained and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV at 222 nm

ColumnPrimesep B, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O – 10/90%
BufferH2SO4 – 0.05%
Flow Rate1.0 ml/min
DetectionUV 220 nm
Samples1.0 mg/ml in MeCN/H2O – 50/50%
Injection volume1 µl
LOD*7 ppb
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Isoxazoles
Analyzing CompoundsHymexazol

Application Column

Primesep B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Hymexazol
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Hymexazol on Newcrom R1 HPLC column

February 16, 2018
Separation of Hymexazol on Newcrom C18 HPLC column

Hymexazol can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Hymexazol
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.