HPLC Determination of Sodium Dodecyl Sulfate on Newcrom BH and Newcrom B Columns

HPLC Method for Sodium dodecyl sulfate on Newcrom B by SIELC Technologies

Sodium dodecyl sulfate (SDS), or sodium lauryl sulfate (SLS), is a surfactant used in cleaning, hygienic, and food products. It has a chemical formula C12H25NaSO4, meaning it has twelve carbon hydrophobic tail attached to a polar sulfate. At low concentrations, it is used as a whipping aid and emulsifier in foods containing egg whites. It has a similar use in pharmaceutics as an ionic solubilizer and, also, an emulsifier. In laboratory uses, it is used as a component for lysing cells during RNA extraction or DNA extraction. You can find detailed UV spectra of Sodium dodecyl sulfate and information about its various lambda maxima by visiting the following link.

Sodium dodecyl sulfate can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate buffer. Detection is performed using UV.

Condition
Column Newcrom B, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 70/30%
Buffer AmFm pH 3.0 – 60 mM
Flow Rate 1.0 ml/min
Detection CAD (Corona) MS- compatible mobile phase

Description
Class of Compounds
 Hydrophobic, Base, Ionizable, Anionic surfactant
Analyzing Compounds Sodium Dodecyl Sulfate

Application Column

Newcrom B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Sodium dodecyl sulfate
Application Detection:
CAD Detection
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