HPLC Method for Serotonin on Primesep 200 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Serotonin.
Serotonin is a neurotransmitter derived from tryptophan with the chemical formula C₁₀H₁₂N₂O. Serotonin is a chemical messenger that is crucial for bodily functions. It helps regulate mood and reduce anxiety, digestion, blood clotting, and contributes to the sleep-wake cycle. Low serotonin levels are often associated with a variety of mental illnesses including but not limited to Obsessive Compulsive Disorder (OCD), Post-Traumatic Stress Disorder (PTSD), Depression, and Anxiety.
Serotonin can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate buffer. Detection is performed using UV.
| Column | Primesep 200, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | AmFm pH 3.0- 20 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 275 nm, MS-compatible mobile phase |
| Class of Compounds |
Drug, Hydrophilic, Supplements, Monoamine, Neurotransmitter |
| Analyzing Compounds | Serotonin |
Application Column
Primesep 200
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
