HPLC Method for Lactic Acid, Lactic Acid Oligomers on Chromni by SIELC Technologies
Lactic acid (milk acid) is a natural organic acid that plays an important role in biochemical processes. It has a carboxylic and hydroxy- groups which, upon standing, can react with each other and form dimers, trimers and higher oligomers. Lactic acid is a polar molecule that does not retain on reversed-phase columns. Lactates can be monitored by low ELSD, CAD or LC/MS.
| Column | Chromni, 3.2 x 150 mm, 3 µm, 100 A, surface coated |
| Mobile Phase | MeCN/H2O – 90/10% |
| Buffer | Ammonium acetate pH 5.0 – 10 mM |
| Flow Rate | 0.5 ml/min |
| Detection | MS ESI SIM 1. [4M-3H2O-H]–– 305 2. [3M-2H2O-H]–– 233 3. [2M-H2O-H]–-161 4. [M−H]⁻- 89 |
| Class of Compounds | Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Lactic Acid, Lactic Acid Oligomers |
Application Column
Chromni
Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: surface coated
Application Analytes:
Lactic AcidLactic Acid Oligomers
Application Detection:
LC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
