HPLC Method for Analysis of Itraconazole on Newcrom R1 Column

HPLC Method for Analysis of Itraconazole on Newcrom R1 Column by SIELC Technologies

HPLC Method for Analysis of Itraconazole on Newcrom R1 Column
HPLC Method for Analysis of Itraconazole on Newcrom R1 Column by SIELC Technologies

Itraconazole is an antifungal medication with the chemical formula C35H38Cl2N8O4. It is used to treat a variety of fungal infections. including but not limited to: Aspergillosis, Blastomycosis, Histoplasmosis, Onychomycosis (fungal infection of the nails), and more. Itraconazole works by inhibiting the synthesis of ergosterol, a critical component of fungal cell membranes. This disruption results in increased cellular permeability, leading to leakage of cellular contents and, ultimately, the death of the fungus.

Uses: It is used to treat a wide range of fungal infections, including:

Itraconazole can be retained, and analyzed on a mixed-mode Newcrom R1 column with a mobile phase consisting of water, Acetonitrile (MeCN), and sulfuric acid. This analytical method can be detected with high resolution and peak symmetry at a wavelength of 200 nm using UV detection

Condition

ColumnNewcrom R1, 4.6 x 150 mm, 3 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 45%,
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV 200 nm

Description

Class of CompoundsDiphenyl ethers
Analyzing CompoundsItraconazole

Application Column

Newcrom R1

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Itraconazole
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.