HPLC Determination of Hydroxychloroquine on Newcrom A Column

HPLC Method for Hydroxychloroquine on Newcrom A by SIELC Technologies

 

High Performance Liquid Chromatography (HPLC) Method for Analysis of Hydroxychloroquine.

Hydroxychloroquine is a prescription medication with the chemical formula C18H26ClN3O. It is used to treat and prevent acute attacks of malaria in adults and children. It is not used in severe or complicated cases. Besides malaria, Hydroxychloroquine is also used to treat discoid lupus erythematosus (DLE), systemic lupus erythematosus (SLE), and chronic rheumatoid arthritis. It is considered unsafe to take this medication alongside other medications.

Hydroxychloroquine can be retained and analyzed using the Newcrom A stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a Ammonium Formate buffer. Detection is performed using UV.

Condition
Column Newcrom A, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 50/50%
Buffer AmFm pH 3.0- 40 mM
Flow Rate 1.0 ml/min
Detection UV 256 nm,  MS-compatible mobile phase

Description
Class of Compounds Hydrophobic, Drug
Analyzing Compounds Hydroxychloroquine

Application Column

Newcrom A

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Hydroxychloroquine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.