HPLC Determination of Docusate Sodium in Softgels on Newcrom BH Column

HPLC Method for Docusate sodium, Dioctyl sulfosuccinic acid on Newcrom BH by SIELC Technologies

 

High Performance Liquid Chromatography (HPLC) Method for Analysis of Docusate.

Docusate is a laxative and stool softener with the chemical formula  C20H37NaO7S. Docusate retains water in the stool, thereby acting as a stool softener to facilitate stool passage. It is found in a variety of forms such as tablets, solution, and syrup over the counter, meaning it can be purchased without a prescription.

Docusate can be retained on the Newcrom BH mixed-mode column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and sulphuric acid (H2SO4) buffer. The analysis method can be UV detected at 200 nm.

Condition
Column Newcrom BH, 3.2 x 100 mm, 3 µm, 100 A, dual ended
Mobile Phase MeCN/H2O -75/25%
Buffer  H2SO4 – 0.5%
Flow Rate 0.5 ml/min
Detection UV, 200 nm

 

Description
Class of Compounds
 Drug
Analyzing Compounds Docusate sodium, Dioctyl sulfosuccinic acid

 

Application Column

Newcrom BH

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Dioctyl sulfosuccinic acid
Docusate sodium
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.