HPLC Method for Analysis of Brilliant Green on Primesep 100 Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Brilliant Green on Primesep 100 by SIELC Technologies.

HPLC Method for Analysis of Brilliant Green on Primesep 100 Column
HPLC Method for Analysis of Brilliant Green on Primesep 100 Column by SIELC Technologies

Brilliant green, also known as zelyonka and zelenka, is a triarylmethane dye with the chemical formula C27H33N2.HO4S. A dilute alcoholic solution of Brilliant Green is used as a topical antiseptic. At lower solutions, it can also be used for mucous membranes and for infants. It is a visible light-activated photocatalyst in organic synthesis

Brilliant Green can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple isocratic mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer.  This analysis method can be UV detected at 600 nm with high resolution and peak symmetry.

Condition

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 80/20%
BufferH3PO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV, 600 nm
Peak Retention Time7.22 min

Description

Class of CompoundsDyes
Analyzing CompoundsBrilliant Green

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Brilliant Green
Application Detection:
Vis Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.