Brilliant Green

Brilliant Green structural formula

CAS Number633-03-4
Molecular FormulaC27H34N2O4S
Molecular Weight482.641
InChI KeyNNBFNNNWANBMTI-UHFFFAOYSA-M
LogP3.18
Synonyms
  • Brilliant Green
  • 4-{[4-(Diethylamino)phenyl](phenyl)methylidene}-N,N-diethylcyclohexa-2,5-dien-1-iminium hydrogen sulfate
  • 633-03-4
  • Ethanaminium, N-[4-[[4-(diethylamino)phenyl]phenylmethylene]-2,5-cyclohexadien-1-ylidene]-N-ethyl-, sulfate (1:1)
  • C.I.Basic Green 1
  • Malachite Green G
  • [4-[4-(Diethylamino)benzhydrylen]cyclohexa-2,5-dien-1-yliden]diethylammoniumhydrogensulfat
  • [4-[4-(diethylamino)benzhydrylene]cyclohexa-2,5-dien-1-ylidene]diethylammonium hydrogen sulphate
  • 12415 Green
  • ADC Brilliant Green Crystals
  • Aizen Diamond Green GH
  • Aizen Malachite Green GH
  • Astra Diamond Green GX
  • Astrazon Green D
  • Avon Green A 4379
  • Basic Bright Green
  • Basic Bright Green Sulfate
  • Basic Brilliant Green
  • Basic Green 1
  • Basic Green V
  • Brilliant green aseptic
  • Brilliant Green B
  • Brilliant Green BP
  • Brilliant Green BP Crystals
  • Brilliant Green BPC
  • Brilliant Green Crystals
  • Brilliant Green Crystals H
  • Brilliant Green DSC
  • Brilliant Green G
  • Brilliant Green GX
  • Brilliant Green Lake
  • Brilliant Green P
  • Brilliant Green Special
  • Brilliant Green Sulfate
  • Brilliant Green WP Crystals
  • Brilliant Green Y
  • Brilliant Green YN
  • Brilliant Green YNS
  • Brilliant Lake Green Y
  • C.I. basic green 001
  • C.I. BASIC GREEN 1
  • Calcozine Brilliant Green G
  • Deorlene Green JJO
  • Diamond Green G
  • Diamond Green GH
  • Ethyl green
  • Fast Green J
  • Fast Green JJO
  • Green EN
  • Hidaco Brilliant Green
  • hidrogenosulfato de [4-[4-(dietilamino)bencidrilen]ciclohexa-2,5-dien-1-iliden]dietilamonio
  • Hydrogenosulfate de [4-[4-(diethylamino)benzhydrylene]cyclohexa-2,5-dien-1-ylidene]diethylammonium
  • hydrogenosulfate de [4-[4-(diethylamino)benzhydrylene]cyclohexa-2,5-diene-1-ylidene]diethylammonium
  • Mitsui Brilliant Green GX
  • NSC 5011
  • Resplendency Green
  • Solid Green
  • Tertrophene Brilliant Green G
  • Tokyo Aniline Brilliant Green
  • Avon Green A-4379
  • Brilliant Green B.P.
  • Brilliant Tungstate Green Toner GT-288
  • EINECS 211-190-1
  • Emerald Green
  • N,N,N',N'-Tetraaethyl-monoaminofuchsoniumhydrogensulfat
  • Brilliant-grun
  • Zelen brilantni
  • Zelen malachitova G
  • Zelen smaragdova
  • Zelen zasadita 1
  • UNII-G0L543D370
  • (4-(4-(Diethylamino)benzhydrylene)cyclohexa-2,5-dien-1-ylidene)diethylammonium hydrogen sulfate
  • NSC-5011
  • (4-(4-(Diethylamino)benzhydrylene)cyclohexa-2,5-dien-1-ylidene)diethylammonium hydrogen sulphate
  • C.I. 42040
  • 1051370-53-6
  • 56730-57-5
  • 70165-37-6
  • 820975-89-1
  • 98712-72-2

Applications:

HPLC Method for Analysis of Brilliant Green on Primesep 100 Column

December 7, 2022

High Performance Liquid Chromatography (HPLC) Method for Analysis of Brilliant Green on Primesep 100 by SIELC Technologies.

HPLC Method for Analysis of Brilliant Green on Primesep 100 Column
HPLC Method for Analysis of Brilliant Green on Primesep 100 Column by SIELC Technologies

Brilliant green, also known as zelyonka and zelenka, is a triarylmethane dye with the chemical formula C27H33N2.HO4S. A dilute alcoholic solution of Brilliant Green is used as a topical antiseptic. At lower solutions, it can also be used for mucous membranes and for infants. It is a visible light-activated photocatalyst in organic synthesis

Brilliant Green can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple isocratic mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer.  This analysis method can be UV detected at 600 nm with high resolution and peak symmetry.

Condition

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 80/20%
BufferH3PO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV, 600 nm
Peak Retention Time7.22 min

Description

Class of CompoundsDyes
Analyzing CompoundsBrilliant Green

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Brilliant Green
Application Detection:
Vis Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Brilliant Green on Newcrom R1 HPLC column

February 16, 2018
Separation of Brilliant Green on Newcrom C18 HPLC column

Brilliant Green can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Brilliant Green
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.