HPLC Method for Azithromycin on Primesep SB by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Azithromycin.
Azithromycin is an antibiotic used in the treatment of various bacterial infections. It is on the WHOs list of essential medicines and is classified as critically important. In reversed-phase HPLC, it generally exhibits significant tailing. The tailing can be substantially reduced by using a mixed-mode Primesep SB column with a simple MS-compatible mobile phase of water, acetonitrile (ACN) and ammonium formate (AmFm) buffer.
| Column | Primesep SB, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 15/85% |
| Buffer | AmFm pH 3.0 – 50 mM |
| Flow Rate | 1.0 ml/min |
| Detection | CAD |
| Class of Compounds |
Drug, Antibiotics |
| Analyzing Compounds | Azithromycin |
Application Column
Primesep SB
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
AzithromycinApplication Detection:
CAD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
