HPLC Method for Alizarin, Alizarin Red S on Newcrom BH by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Alizarin, Alizarin Red S.
Alizarin and Alizarin Red S are both dyes with applications in locating calcium deposits in tissues. They differ in Alizarin Red S having an additional sulfonic acid group. They can be separated in HPLC using a Newcrom BH mixed-mode column using a mobile phase of methanol (MeOH) and water and sulfuric acid (H2SO4) as a buffer. UV detection at 250nm.
| Column | Newcrom BH, 4.6 x 50 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeOH/H2O – 75/25% |
| Buffer | H2SO4 – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 250 nm |
| Class of Compounds | Acid, Dyes |
| Analyzing Compounds | Alizarin, Alizarin Red S |
Application Column
Newcrom BH
Column Diameter: 4.6 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
AlizarinAlizarin Red S
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
