Alizarin Red S

CAS Number130-22-3
Molecular FormulaC14H7NaO7S
Molecular Weight342.26
InChI KeyHFVAFDPGUJEFBQ-UHFFFAOYSA-M
Synonyms
  • Alizarin Red S
  • 130-22-3
  • Sodium alizarinesulfonate
  • sodium 3,4-dihydroxy-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
  • Mordant Red 3
  • Alizarine S
  • Acid Red Alizarine
  • Sodium alizarin-3-sulfonate
  • Alizarine Red S sodium salt
  • Alizarinsulfonate
  • Alizarin S
  • Alizarine Red A
  • Alizarine Red W
  • C.I. 58005
  • Fenakrom Red W
  • Diamond Red W
  • Alizarine Red AS
  • Alizarine Red SW
  • Alizarine Red SZ
  • Alizarine Red WA
  • Alizarine Red WS
  • Ahcoquinone Red S
  • Alizarinrot-S [German]
  • Alizarin-3-sulfonate sodium salt
  • Carnelio Rubine Lake
  • Chrome Red Alizarine
  • Oxanal Fast Red SW
  • UNII-3F3AT0Q12H
  • Ext D and C Red No. 7
  • Sodium alizarinsulfonate
  • Alizarine Red Indicator
  • Alizarine Red For Wool
  • Mitsui Alizarine Red S
  • C.I. Mordant Red 3
  • Alizarin Carmine
  • Alizarine S Extra Pure A
  • Alizarine Carmine Indicator
  • Alizarine S Extra Conc. A Export
  • Alizarin Carmine (biological stain)
  • Alizarin sulfonate sodium
  • Alizarine Red S (biological stain)
  • Sodium 3,4-dihydroxyanthraquinone-2-sulfonate
  • Calcochrome Alizarine Red SC
  • EINECS 204-981-8
  • Alizarin Red S (C.I. 58005)
  • Alizarin Red S (sodium)
  • Alizarine Carmine (Biological stain)

Applications:

Uv-Vis Spectrum of Alizarin Red

April 8, 2026

Access the UV-Vis Spectrum SIELC Library

If you are looking for optimized HPLC method to analyze Alizarin Red S check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase.  The compound was dissolved with water. This analysis was done with the mobile phase made of 20% acetonitrile and 80% water.

 

 

Application Analytes:
Alizarin Red S
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Alizarin and Alizarin Red S on Newcrom BH Column

July 9, 2020

HPLC Method for Alizarin, Alizarin Red S on Newcrom BH by SIELC Technologies

 

High Performance Liquid Chromatography (HPLC) Method for Analysis of Alizarin, Alizarin Red S.

Alizarin and Alizarin Red S are both dyes with applications in locating calcium deposits in tissues. They differ in Alizarin Red S having an additional sulfonic acid group. They can be separated in HPLC using a Newcrom BH mixed-mode column using a mobile phase of methanol (MeOH) and water and sulfuric acid (H2SO4) as a buffer. UV detection at 250nm.

Condition
Column Newcrom BH, 4.6 x 50 mm, 5 µm, 100 A, dual ended
Mobile Phase MeOH/H2O – 75/25%
Buffer H2SO4 – 0.5%
Flow Rate 1.0 ml/min
Detection UV 250 nm

Description
Class of Compounds Acid, Dyes
Analyzing Compounds Alizarin, Alizarin Red S

 

Application Column

Newcrom BH

Column Diameter: 4.6 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Alizarin
Alizarin Red S
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.