CAS Number | 130-22-3 |
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Molecular Formula | C14H7NaO7S |
Molecular Weight | 342.26 |
InChI Key | HFVAFDPGUJEFBQ-UHFFFAOYSA-M |
Synonyms |
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Applications:
HPLC Separation of Alizarin and Alizarin Red S on Newcrom BH Column
July 9, 2020
HPLC Method for Alizarin, Alizarin Red S on Newcrom BH by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Alizarin, Alizarin Red S.
Alizarin and Alizarin Red S are both dyes with applications in locating calcium deposits in tissues. They differ in Alizarin Red S having an additional sulfonic acid group. They can be separated in HPLC using a Newcrom BH mixed-mode column using a mobile phase of methanol (MeOH) and water and sulfuric acid (H2SO4) as a buffer. UV detection at 250nm.
Column | Newcrom BH, 4.6 x 50 mm, 5 µm, 100 A, dual ended |
Mobile Phase | MeOH/H2O – 75/25% |
Buffer | H2SO4 – 0.5% |
Flow Rate | 1.0 ml/min |
Detection | UV 250 nm |
Class of Compounds | Acid, Dyes |
Analyzing Compounds | Alizarin, Alizarin Red S |
Application Column
Newcrom BH
Column Diameter: 4.6 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Alizarin Red S