View on hplc.cloud
Alizarin and Alizarin Red S are both dyes with applications in locating calcium deposits in tissues. They differ in Alizarin Red S having an additional sulfonic acid group. They can be separated in HPLC using a Newcrom BH mixed-mode column using a mobile phase of methanol (MeOH) and water and sulfuric acid (H2SO4) as a buffer. UV detection at 250nm.
| Column | Newcom BH, 4.6x50 mm, 5 µm, 100A |
| Mobile Phase | MeOH/H2O - 75/25% |
| Buffer | H2SO4 - 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 250 nm |
| Class of Compounds | Acid, Dyes |
| Analyzing Compounds | Alizarin, Alizarin Red S |
Application Analytes:
AlizarinAlizarin Red S