HPLC Separation of Alizarin and Alizarin Red S on Newcrom BH Column

HPLC.cloud View on hplc.cloud Alizarin and Alizarin Red S are both dyes with applications in locating calcium deposits in tissues. They differ in Alizarin Red S having an additional sulfonic acid group. They can be separated in HPLC using a Newcrom BH mixed-mode column using a mobile phase of methanol (MeOH) and water and sulfuric acid (H2SO4) as a buffer. UV detection at 250nm.  


Column Newcom BH, 4.6x50 mm, 5 µm, 100A
Mobile Phase MeOH/H2O - 75/25%
Buffer H2SO4 - 0.5%
Flow Rate 1.0 ml/min
Detection UV 250 nm


Class of Compounds Acid, Dyes
Analyzing Compounds Alizarin, Alizarin Red S
Application Analytes:
Alizarin Red S