HPLC Determination of 6-aminocaproic Acid on Newcrom A Column

HPLC Method for Analysis of 6-Aminocaproic acid on Newcrom A by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of 6-Aminocaproic acid.

6-Aminocaproic Acid, brand name Amicar, is a clotting agent used to control excessive bleeding in patients during or after surgery by acting as an inhibitor to fibrinolysis. It is also used to treat  a variety of disorders from Thrombocytopenia to bleeding disorders. It’s chemical formula is C6H13NO2. You can find detailed UV spectra of 6-Aminocaproic Acid and information about its various lambda maxima by visiting the following link.

It can be retained using HPLC and low capacity Newcrom A mixed-mode column using isocratic analysis with mobile phase consisting of acetonitrile and water (ACN/H2O) and MS-compatible ammonium formate (AmFm) buffer.

ColumnNewcrom A, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 10/90%
BufferAmmonium Formate pH 3.0 – 10 мМ
Flow Rate1.0 ml/min
DetectionCAD, Corona (MS-compatible mobile phase)
Class of CompoundsAcid
Analyzing Compounds6-Aminocaproic acid

Application Column

Newcrom A

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
6-Aminocaproic acid
Application Detection:
CAD Detection
Corona Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.