HPLC Method for Analysis of 4-Aminophenol on Primesep 100 by SIELC Technologies
4-Aminophenol, also known as para-aminophenol, and Rodinal is a popular film developing compound and building block in organic chemistry reactions. Most notably, it is a key compound used in the production of acetaminophen (also known as paracetamol and Tylenol). It has the chemical formula H2NC6H4OH. It is usually found as a white, moderately soluble in alcohol, powder, but it can be recrystallized in hot water. You can find detailed UV spectra of 4-Aminophenol and information about its various lambda maxima by visiting the following link.
4-Aminophenol, 4-Aminophenol hydrochloride can be separated, retained, and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and an sulfuric acid (H2SO4) or Perchloric acid (HClO4)buffer. This analysis method can be detected in the UV regime at 275 nm.
Condition
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 10% |
| Buffer | H2SO4 – 0.2% or HClO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200, 210, 275 nm |
| Peak Retention Time | 3.7 min, 6.9 |
Description
| Class of Compounds | Alcohols, Phenols, Amines, Amine Salts |
| Analyzing Compounds | 4-Aminophenol, 4-Aminophenol hydrochloride |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
4-Aminophenol hydrochloride
