HPLC Method for Analysis of Cyclosporine H on Legacy L16 Column

HPLC Method for Analysis of Cyclosporin H on Legacy L16 by SIELC Technologies

HPLC Method for Analysis of Cyclosporin H in on Legacy L16 Column

Cyclosporine H is a selective inhibitor of formyl peptide receptor-1 (FPR-1) with the molecular formula C61H111N11O12. It is used primarily used in research. Unlike Cyclosporin A, B, and C, it does not have immunosuppressive properties. You can find detailed UV spectra of Cyclosporine H and information about its various lambda maxima by visiting the following link.

Cyclosporin H can be retained and analyzed using the Legacy L16 stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of acetonitrile, water, methanol, and phosphoric acid. Detection is performed using UV.

Condition

ColumnLegacy L16, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/MeOH/H2O – 50/5/45%
BufferH3PO4 – 0.05%
Flow Rate1.0 ml/min
DetectionUV 210
Column Temperature70 C

Description

Class of CompoundsInhibitor
Analyzing CompoundsCyclosporin H

Application Column

SIELC Legacy L16 HPLC column

Legacy L16

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Cyclosporin H
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.