HPLC Analysis of Caffeine in Drinks with Environmentally Friendly Mobile Phase on Primesep SB Column

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Column Primesep SB, 3.2×100 mm, 5 µm, 100A
Mobile Phase EtOH – 10%
Buffer H3PO4 – 0.3%
Flow Rate 0.5 ml/min
Detection UV, 270 nm


Class of Compounds
Xanthine, Hydrophobic, Ionizable
Analyzing Compounds Caffeine


Application Column

Primesep SB

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.