HPLC Method for Analysis of Blasticidin S on BIST B Column

HPLC Method for Blasticidin-S on BIST B by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Blasticidin-S

Blasticidin S is an antibiotic with the chemical formula C17H26N8O5. It is primarily used in biology research for selecting cells of interest among cell cultures. It works through disrupting protein translation within human cells, fungi, and bacteria due to it’s resemblance to cytidine. You can find detailed UV spectra of Blasticidin-S and information about its various lambda maxima by visiting the following link.

Blasticidin-S can be retained and analyzed using the BIST B stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of acetonitrile (MeCN). Detection is performed using UV.

ColumnBIST B, 4.6 x 50 mm, 5 µm, 100 A, dual ended
Mobile PhaseGradient MeCN/H2O – 80/20 – 50/50 %, 10 min
BufferSulfuric Acid – 0.2%
Flow Rate1.0 ml/min
DetectionUV 275nm
Limit of Detection*13 ppb
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
Class of Compounds
Antibiotic
Analyzing CompoundsBlasticidin-S

Application Column

BIST B

Column Diameter: 4.6 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Blasticidin-S
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.