| CAS Number | 2079-00-7 |
|---|---|
| Molecular Formula | C17H26N8O5 |
| Molecular Weight | 422.447 |
| InChI Key | CXNPLSGKWMLZPZ-ZNIXKSQXSA-N |
| LogP | -2.96 |
| Synonyms |
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Applications:
Uv-Vis Spectrum of Blasticidin S
May 26, 2026
Access the UV-Vis Spectrum SIELC Library
If you are looking for optimized HPLC method to analyze Blasticidin-S check our HPLC Applications library
UV/Vis.: λmax: 196, 278 nm
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The compound was dissolved in acetonitrile. This analysis was done with the mobile phase made of acetonitrile.
HPLC UV Method for Analysis of Blasticidine S on Chromni Column
May 26, 2026
HPLC Method for Blasticidin-S on Chromni by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Blasticidin-S
Blasticidin S is an antibiotic with the chemical formula C17H26N8O5. It is primarily used in biology research for selecting cells of interest among cell cultures. It works through disrupting protein translation within human cells, fungi, and bacteria due to it’s resemblance to cytidine. You can find detailed UV spectra of Blasticidin-S and information about its various lambda maxima by visiting the following link.
Blasticidin-S can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an isocraticmethod with a simple mobile phase consisting of acetonitrile (MeCN). Detection is performed using UV.
| Column | Chromni, 4.6 x 150 mm, 3 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 70/30 |
| Buffer | Ammonium Formate pH 3.0 – 20 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 275nm |
| Limit of Detection* | 3.1 ppb |
| Class of Compounds | Antibiotic |
| Analyzing Compounds | Blasticidin-S |
Application Column
Chromni
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended
HPLC Method for Analysis of Blasticidin S on BIST B Column
May 26, 2026
HPLC Method for Blasticidin-S on BIST B by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Blasticidin-S
Blasticidin S is an antibiotic with the chemical formula C17H26N8O5. It is primarily used in biology research for selecting cells of interest among cell cultures. It works through disrupting protein translation within human cells, fungi, and bacteria due to it’s resemblance to cytidine. You can find detailed UV spectra of Blasticidin-S and information about its various lambda maxima by visiting the following link.
Blasticidin-S can be retained and analyzed using the BIST B stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of acetonitrile (MeCN). Detection is performed using UV.
| Column | BIST B, 4.6 x 50 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | Gradient MeCN/H2O – 80/20 – 50/50 %, 10 min |
| Buffer | Sulfuric Acid – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 275nm |
| Limit of Detection* | 13 ppb |
| Class of Compounds | Antibiotic |
| Analyzing Compounds | Blasticidin-S |
Application Column
BIST B
Column Diameter: 4.6 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Separation of Blasticidin-S on Newcrom R1 HPLC column
February 16, 2018
Blasticidin-S can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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