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For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.

HPLC Method for Meglumine (N-Methyl-D-glucamine), Flunixin on Primesep 100 by SIELC Technologies

Flunixin is an analgesic and antipyretic nonsteroidal anti-inflammatory drug (NSAID) with the chemical formula C₁₄H₁₁F₃N₂O₂. It is used primarily by veterinarians in bovine and equine treatments. Prolonged consecutive use can cause health problems due to toxicity. Due to that, it is a prohibited substance under International Federation for Equestrian Sports rules. It is typically produced as a Meglumine salt, which helps stabilize and bulk up the medication in its solid form. You can find detailed UV spectra of Flunixin and information about its various lambda maxima by visiting the following link.

Meglumine is a sugar alcohol with the chemical formula C₇H₁₇NO₅. It is a derivative of glucose that is used primarily as an excipient in pharmaceuticals. You can find detailed UV spectra of Meglumine and information about its various lambda maxima by visiting the following link.

Meglumine (N-Methyl-D-glucamine), Flunixin can be retained, separated, and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple isocratic mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer.  This analysis method can be UV detected at 252 nm (Flunixin) with high resolution and peak symmetry and is compatible with Mass Spectrometry (MS), ELSD, and CAD.

HPLC Method for Flunixin on Primesep 100 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Flunixin

Flunixin is an analgesic and antipyretic nonsteroidal anti-inflammatory drug (NSAID) with the chemical formula C₁₄H₁₁F₃N₂O₂. It is used primarily by veterinarians in bovine and equine treatments. Prolonged consecutive use can cause health problems due to toxicity. Due to that, it is a prohibited substance under International Federation for Equestrian Sports rules. It is typically produced as a Meglumine salt, which helps stabilize and bulk up the medication in its solid form. You can find detailed UV spectra of Flunixin and information about its various lambda maxima by visiting the following link.

Flunixin can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.