| CAS Number | 141-97-9 |
|---|---|
| Molecular Formula | C6H10O3 |
| Molecular Weight | 130.144 |
| InChI Key | XYIBRDXRRQCHLP-UHFFFAOYSA-N |
| LogP | 0.25 |
| Synonyms |
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Applications:
HPLC Method for Analysis of Ethyl acetoacetate on Newcrom R1 Column
June 9, 2023
HPLC Method for Analysis of Ethyl acetoacetate on Newcrom R1 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
Ethyl acetoacetate (EAA) is an organic compound used in various applications, including as a starting material in organic synthesis and as a flavoring agent. It’s a type of chemical compound known as an ester, which means it contains a carbonyl group adjacent to an ether group.
In terms of structure, EAA contains two main parts: an ethyl group (a carbon and two hydrogens, CH3CH2-) and an acetoacetate group (CH3C(=O)CH2C(=O)O-).
In organic chemistry, EAA is known for its role in the Claisen condensation and other reactions where it behaves as an active methylene compound. An active methylene compound is a compound that contains a methylene group (CH2) adjacent to one or more electron-withdrawing groups, such as a carbonyl group, which can make the hydrogen atoms on the methylene group acidic and easily removed.
As a flavoring agent, it’s used in foods and beverages to impart apple and pear flavors.
Ethyl acetoacetate can be retained and analyzed on a reverse-phase Newcrom R1, 3.2 x 100 mm, 5 µm, 100 A column with a mobile phase consisting of water and Acetonitrile (MeCN). This analysis method can be detected with an Evaporative Light Scattering Detector (ELSD) or any other evaporative detection method (CAD, ESI-MS).
LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ethyl acetoacetate
Condition
| Column | Newcrom R1, 3.2 x 100 mm, 5 µm, 100 A |
| Mobile Phase | MeCN/H2O -20/80% |
| Buffer | None |
| Flow Rate | 0.5 ml/min |
| Detection | UV 200 nm |
| Peak Retention Time | 3.62 min |
| Sample concentration | 0.04 mg/ml |
| Injection volume | 1 µl |
| Sample diluent | MeCN/H2O -20/80% |
| LOD | 6 ppb |
Description
| Class of Compounds | Beta-keto esters, Esters |
| Analyzing Compounds | Ethyl acetoacetate |
Application Column
Newcrom R1
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Separation of Ethyl acetoacetate on Newcrom R1 HPLC column
February 16, 2018
Ethyl acetoacetate can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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