HPLC Method for Analysis of Ethyl acetoacetate on Newcrom R1 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
Ethyl acetoacetate (EAA) is an organic compound used in various applications, including as a starting material in organic synthesis and as a flavoring agent. It’s a type of chemical compound known as an ester, which means it contains a carbonyl group adjacent to an ether group.
In terms of structure, EAA contains two main parts: an ethyl group (a carbon and two hydrogens, CH3CH2-) and an acetoacetate group (CH3C(=O)CH2C(=O)O-).
In organic chemistry, EAA is known for its role in the Claisen condensation and other reactions where it behaves as an active methylene compound. An active methylene compound is a compound that contains a methylene group (CH2) adjacent to one or more electron-withdrawing groups, such as a carbonyl group, which can make the hydrogen atoms on the methylene group acidic and easily removed.
As a flavoring agent, it’s used in foods and beverages to impart apple and pear flavors.
Ethyl acetoacetate can be retained and analyzed on a reverse-phase Newcrom R1, 3.2 x 100 mm, 5 µm, 100 A column with a mobile phase consisting of water and Acetonitrile (MeCN). This analysis method can be detected with an Evaporative Light Scattering Detector (ELSD) or any other evaporative detection method (CAD, ESI-MS).
LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ethyl acetoacetate
Condition
| Column | Newcrom R1, 3.2 x 100 mm, 5 µm, 100 A |
| Mobile Phase | MeCN/H2O -20/80% |
| Buffer | None |
| Flow Rate | 0.5 ml/min |
| Detection | UV 200 nm |
| Peak Retention Time | 3.62 min |
| Sample concentration | 0.04 mg/ml |
| Injection volume | 1 µl |
| Sample diluent | MeCN/H2O -20/80% |
| LOD | 6 ppb |
Description
| Class of Compounds | Beta-keto esters, Esters |
| Analyzing Compounds | Ethyl acetoacetate |
Application Column
Newcrom R1
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
