| CAS Number | 3282-73-3 |
|---|---|
| Molecular Formula | C26H56BrN |
| Molecular Weight | 462.6 |
| InChI Key | XRWMGCFJVKDVMD-UHFFFAOYSA-M |
| Synonyms |
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Applications:
Uv-Vis Spectrum of Didodecyldimethylammonium bromide
April 2, 2026
Access the UV-Vis Spectrum SIELC Library

If you are looking for optimized HPLC method to analyze Didodecyldimethylammonium bromide check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. This analysis was done with the mobile phase made of 20% acetonitrile and 80% water.
HPLC Determination of Didodecyldimethylammonium Bromide on Primesep SB Column
December 16, 2019
HPLC Method for Didodecyldimethylammonium bromide, Bromide on Primesep SB by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Didodecyldimethylammonium bromide, Bromide
Didodecyldimethylammonium Bromide is a double-chain cationic surfactant with the C26H56BrN molecular structure. It is occasionally used as a disinfectant, synthesis of nanoparticles and organoclays. Neuroblastoma and leukemia cells are more sensitive to it than to any other carcinoma cells.
Didodecyldimethylammonium bromide, Bromide can be retained and analyzed using the Primesep SB stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a Ammonium Formate buffer. Detection is performed using CAD.
| Column | Primesep SB, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 60/40% |
| Buffer | AmFm pH 3.0 – 40 mM |
| Flow Rate | 1.0 ml/min |
| Detection | CAD (Corona) (MS-compatible mobile phase) |
| Class of Compounds | Base, Ions, Hydrophobic, Ionizable |
| Analyzing Compounds | Didodecyldimethylammonium bromide, Bromide |
Application Column
Primesep SB
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Didodecyldimethylammonium bromide


