| CAS Number | 439-14-5 |
|---|---|
| Molecular Formula | C16H13ClN2O |
| Molecular Weight | 284.740 |
| InChI Key | AAOVKJBEBIDNHE-UHFFFAOYSA-N |
| LogP | 2.82 |
| Synonyms |
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Applications:
HPLC Determination of Diazepam on Newcrom R1 Column
March 29, 2021
Separation type: Liquid Chromatography Reverse Phase
High Performance Liquid Chromatography (HPLC) Method for Analysis of DiazepamDiazepam (Valium) is a benzodiazepine.
It is thought that benzodiazepines work by enhancing the activity of certain neurotransmitters in the brain. Diazepam increases the activity of gamma-aminobutyric acid (GABA), a special chemical that can send signals throughout the nervous system. Diazepam can be retained in HPLC on Newcrom R1 reverse-phase column with the simple isocratic mobile phase consisting of acetonitrile (MeCN) and water. The analysis method can be UV detected at 240nm.
| Column | Newcrom R1, 2.1×100 mm, 5 µm, 100A |
| Mobile Phase | MeCN – 30% |
| Buffer | No |
| Flow Rate | 0.2 ml/min |
| Detection | 240 nm |
| Class of Compounds | Drug, Hydrophobic |
| Analyzing Compounds | Diazepam |
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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Separation of Diazepam on Newcrom R1 HPLC column
February 16, 2018
Diazepam can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options