HPLC Determination of Diazepam on Newcrom R1 Column

Separation type: Liquid Chromatography Reverse Phase

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High Performance Liquid Chromatography (HPLC) Method for Analysis of DiazepamDiazepam (Valium) is a benzodiazepine.

It is thought that benzodiazepines work by enhancing the activity of certain neurotransmitters in the brain. Diazepam increases the activity of gamma-aminobutyric acid (GABA), a special chemical that can send signals throughout the nervous system. Diazepam can be retained in HPLC on Newcrom R1 reverse-phase column with the simple isocratic mobile phase consisting of acetonitrile (MeCN) and water. The analysis method can be UV detected at 240nm.


Column Newcrom R1, 2.1x100 mm, 5 µm, 100A
Mobile Phase MeCN - 30%
Buffer No
Flow Rate 0.2 ml/min
Detection 240 nm


Class of Compounds Drug, Hydrophobic
Analyzing Compounds Diazepam
Application Analytes: