HPLC Method for Diazepam on Newcrom R1 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Diazepam.
Diazepam, also known as Valium, is a benzodiazepine. It is thought that benzodiazepines work by enhancing the activity of certain neurotransmitters in the brain. Diazepam increases the activity of gamma-aminobutyric acid (GABA), a special chemical that can send signals throughout the nervous system.
Diazepam can be retained in HPLC on Newcrom R1 reverse-phase column with the simple isocratic mobile phase consisting of acetonitrile (MeCN) and water. The analysis method can be UV detected at 240nm.
| Column | Newcrom R1, 2.1 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 30% |
| Buffer | No |
| Flow Rate | 0.2 ml/min |
| Detection | 240 nm |
| Class of Compounds | Drug, Hydrophobic |
| Analyzing Compounds | Diazepam |
Application Column
Newcrom R1
Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
DiazepamApplication Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
