Determination Omeprazole on Obelisc R Column

HPLC Method for Omeprazole on Obelisc R by SIELC Technologies

Condition 1
Column Obelisc R, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN -35%
Buffer Ammonium Acetate  pH 5.5 – 10 mM
Flow Rate 1.0 ml/min
Detection UV 300 nm

Condition 2
Column Obelisc R, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase Gradient MeCN -20-50% in 15 min + 5 min hold
Buffer Ammonium Acetate  pH 5.5 – 10 mM
Flow Rate 1.0 ml/min
Detection UV 300 nm

High Performance Liquid Chromatography (HPLC) Method for Analysis of Omeprazole.

Omeprazole is a proton pump inhibitor (PPI) with the chemical formula C17H19N3O3S. It is a medication that decreases the amount of acid produced in the stomach.  Omeprazole has a bactericidal effect on Helicobacter pylori.

Obelisc R column which has both positive and negative ion-pairs embedded in the stationary phase allows for fine tuning and separation of a wide range of compounds with different ionic properties.  Omeprazole was separated isocratically using a simple MS-compatible mobile phase of acetonitrile (ACN) and water with Ammonium Acetate (AmAc) buffer.  Can also be UV detected at 300nm.

Description
Class of Compounds
  Drug
Analyzing Compounds Omeprazole

 

Application Column

Obelisc R

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Omeprazole
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.