Nicotinamide

Nicotinamide structural formula

CAS Number98-92-0
Molecular FormulaC6H6N2O
Molecular Weight122.127
InChI KeyDFPAKSUCGFBDDF-UHFFFAOYSA-N
LogP-0.370
Synonyms
  • Niacinamide
  • Pyridine-3-carboxamide
  • 3-Pyridinecarboxamide
  • 98-92-0
  • Nicotinamide
  • 3-Pyridinecarboxamide
  • Nam
  • 3-(Aminocarbonyl)pyridine
  • 3-Amidopyridine
  • 3-Carbamoylpyridine
  • 3-Pyridinecarboxylic acid amide
  • Aminicotin
  • Benicot
  • Delonin Amide
  • Dipegyl
  • m-(Aminocarbonyl)pyridine
  • Niavit PP
  • Nicamina
  • Nicamindon
  • Nicasir
  • Nicobion
  • Nicofort
  • Nicosylamide
  • Nicotilamide
  • Nicotinamid
  • nicotinamida
  • Nicotine acid amide
  • Nicotinic acid amide
  • Nicotinic amide
  • Nicotylamide
  • Nicovit
  • Nicovitina
  • Nictoamide
  • Niocinamide
  • Niozymin
  • NSC 13128
  • NSC 27452
  • Papulex
  • Pelmine
  • Pelonin amide
  • PYRIDINE-3-CARBOXAMIDE
  • Vi-Nicotyl
  • Vitamin B
  • β-Pyridinecarboxamide
  • EINECS 202-713-4
  • Pyridine, 3-carbamoyl-
  • Nicotinsaureamid
  • UNII-25X51I8RD4
  • Acid amide
  • Amid kyseliny nikotinove
  • Amide PP
  • Amixicotyn
  • Amnicotin
  • Austrovit PP
  • Dipegyl
  • Dipigyl
  • Endobion
  • Factor pp
  • Hansamid
  • Inovitan PP
  • Mediatric
  • Nandervit-N
  • Niacevit
  • Niamide
  • Nicamide
  • Nicogen
  • Nicomidol
  • Nicosan 2
  • Nicota
  • Nicotamide
  • Nicotililamido
  • Nicotinamidum
  • Nicotine amide
  • Nicotinsaeureamid
  • Nicotinsaureamid
  • Nicotol
  • Nicotylamidum
  • Nicovel
  • Nicovitol
  • Nicozymin
  • Niko-tamin
  • Nikotinamid
  • Nikotinsaeureamid
  • PP-Faktor
  • Pelmin
  • Propamine A
  • Pyridine-3-carboxylic acid amide
  • Savacotyl
  • Vi-noctyl
  • Witamina PP
  • b-Pyridinecarboxamide
  • beta-pyridinecarboxamide
  • niacin
  • vitamin B3
  • 123574-63-0
  • 37321-14-5
  • 78731-47-2

Applications:

HPLC Method for Analysis of Water-Soluble Vitamins (B1, B2, B3, B6) on Primesep 100 Column

August 28, 2024

High Performance Liquid Chromatography (HPLC) Method for Analysis of Water-Soluble Vitamins (B1, B2, B3, B6) on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Vitamin B1 (Thiamine), Vitamin B2 (Riboflavin), Nicotinamide, Vitamin B6 (Pyridoxine), Pyridoxamine, Pyridoxal Phosphate

Water-Soluble Vitamins (B1, B2, B3, B6) can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an gradient method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 300 nm.

You can find detailed UV spectra of Vitamin B1 (Thiamine), Vitamin B2 (Riboflavin), Nicotinamide, Vitamin B6 (Pyridoxine), Pyridoxamine, Pyridoxal Phosphate and information about its various lambda maxima by visiting the following links: Vitamins (B1, B2, B3, B6)

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN – 10%
BufferGradient H2SO4 -0.1-0.5% in 10 min
Flow Rate1.0 ml/min
DetectionUV 270 nm

Class of Compounds
Water-Soluble Vitamins (B1, B2, B3, B6)
Analyzing CompoundsVitamin B1 (Thiamine), Vitamin B2 (Riboflavin), Nicotinamide, Vitamin B6 (Pyridoxine), Pyridoxamine, Pyridoxal Phosphate

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Nicotinamide
Pyridoxal Phosphate
Pyridoxamine
Vitamin B1 (Thiamine)
Vitamin B2 (Riboflavin)
Vitamin B6 (Pyridoxine)

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

UV-Vis Spectrum of Nicotinamide

August 13, 2024
UV-Vis Spectrum of Nicotinamide. Absorption Maxima: 210 nm, 260 nm.

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

Application Analytes:
Nicotinamide
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Nicotinamide and Pyridoxine on Primesep 100 Column

August 13, 2024

High Performance Liquid Chromatography (HPLC) Method for Analysis of Nicotinamide, Vitamin B6 (Pyridoxine) on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

HPLC Method for Analysis of Nicotinamide, Vitamin B6 (Pyridoxine) on Primesep 100 Column


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Nicotinamide, Vitamin B6 (Pyridoxine)

Nicotinamide is a specific form of vitamin B3, an essential nutrient that is crucial for various bodily functions. Along with niacin (nicotinic acid), nicotinamide is one of the primary forms of vitamin B3 that the body uses. Both of these forms are converted into coenzymes NAD+ and NADP+, which play vital roles in energy production, DNA repair, and cellular communication. This conversion is essential for maintaining the body’s metabolic processes, highlighting the importance of nicotinamide as a form of vitamin B3.

Pyridoxine is a form of vitamin B6, which is essential for numerous biochemical processes in the body. Vitamin B6 exists in three natural forms—pyridoxine, pyridoxal, and pyridoxamine—all of which are converted into the active coenzyme pyridoxal 5′-phosphate (PLP). This active form is critical for amino acid metabolism, neurotransmitter synthesis, and hemoglobin production. As a form of vitamin B6, pyridoxine is key to maintaining proper neurological and immune function, ensuring that the body can perform a wide range of essential functions.

Nicotinamide, Vitamin B6 (Pyridoxine) can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 270 nm.

You can find detailed UV spectra of Nicotinamide, Vitamin B6 (Pyridoxine) and information about its various lambda maxima by visiting the following links: UV-Vis Spectrum of Nicotinamide, UV-Vis Spectrum of Pyridoxine.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN – 20%
BufferH2SO4 – 0.1%
Flow Rate1.0 ml/min
DetectionUV 270 nm
Limit of Detection1. 22.1 ppb, 2. 61.1
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Vitamin B
Analyzing CompoundsNicotinamide, Vitamin B6 (Pyridoxine)

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Nicotinamide
Vitamin B6 (Pyridoxine)

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Separation of Nicotinic Acid, Nicotinamide, Tranexamic Acid on Primesep 100 Column

March 28, 2024

HPLC Method for Separation of Nicotinic Acid, Nicotinamide, Tranexamic Acid on Primesep 100 Column

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

HPLC Method for Separation of Nicotinic Acid, Nicotinamide, Tranexamic Acid on Primesep 100 Column
HPLC Method for Analysis of Nicotinic Acid/Niacin (3-pyridinecarboxylic acid), Nicotinamide, Tranexamic acid on Primesep 100 Column by SIELC Technologies


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Nicotinic Acid/Niacin (3-pyridinecarboxylic acid), Nicotinamide, Tranexamic acid

Nicotinic acid and nicotinamide are both forms of vitamin B3, also known as niacin. They play crucial roles in the body’s energy metabolism and are essential for maintaining the health of the skin, nervous system, and digestive system.

  1. Nicotinic Acid (Niacin): This is one of the two principal forms of vitamin B3. Nicotinic acid is commonly used as a dietary supplement to treat vitamin B3 deficiency, and it’s also prescribed in higher doses to help lower cholesterol levels. It works by dilating blood vessels, which can lead to a flushing sensation, hence it is sometimes referred to as “flushing niacin.”
  2. Nicotinamide: Also known as niacinamide, this is the other principal form of vitamin B3. Nicotinamide is a precursor to nicotinamide adenine dinucleotide (NAD+), a coenzyme involved in various cellular processes, including energy metabolism. Nicotinamide is often used in skincare products for its anti-inflammatory properties and its ability to improve the appearance of aging skin and reduce hyperpigmentation.

Tranexamic acid, on the other hand, is a synthetic derivative of the amino acid lysine. It is primarily used as an antifibrinolytic agent, meaning it helps to prevent the breakdown of blood clots. Tranexamic acid is commonly used to treat or prevent excessive bleeding, particularly in conditions such as heavy menstrual bleeding, traumatic hemorrhage, and during surgeries where significant blood loss is expected. Additionally, it has been investigated for its potential role in treating melasma, a common skin condition characterized by hyperpigmentation.

Each of these compounds has distinct biochemical properties and medical applications, but they all play important roles in human health and medicine.

Nicotinic Acid, Nicotinamide, Tranexamic Acid can be retained and separated using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV at 210 nm

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN 40%
BufferH2SO4 – 0.05%
Flow Rate1.0 ml/min
DetectionUV 210nm

Class of Compounds
Acids
Analyzing CompoundsNicotinic Acid/Niacin (3-pyridinecarboxylic acid), Nicotinamide, Tranexamic acid

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Nicotinamide
Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)
Tranexamic acid

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Simultaneous Determination of Nicotinic Acid, Nicotinamide, Pyridine Dicarboxylic Acid, Ethyl Methyl Pyridine and Ethyl Nicotinate on Primesep 100 Column

August 29, 2022

Separation type: Liquid Chromatography Mixed-mode



High Performance Liquid Chromatography (HPLC) Method for Analysis of Nicotinic Acid, Nicotinamide, Pyridine Dicarboxylic Acid, Ethyl Methyl Pyridine and Ethyl Nicotinate Nicotinic acid, also known as Niacin or Vitamin B3, is an essential nutrient that plants and animals synthesize from tryptophan. It is also used as a dietary supplement to treat pellagra. A derivative of Nicotinic acid, Nictotinamide, is a key component of the coenzymes nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP+). Pyridine Dicarboxylic acid (PDA), or Dipicolinic acid (DPA), is a key factor in bacterial endospore heat resistance. It also plays an important role in preparing transition metal complexes for ion chromatography. Ethyl Methyl Pyridine is a common precursor to Nicotinic acid. Ethyl nicotinate, or nicotine acid ethyl ester, is a popular topical treatment for ligament, joint, and muscle pain. These 5 organic compounds can be separated, retained, and analyzed on a Primesep 100 mixed-mode column using an analytical method with a simple gradient mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be UV detected at 250 nm with high resolution and peak symmetry.

Condition

Column Primesep 100, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN – Gradient
Buffer H2SO4 – 0.05%
Flow Rate 1.0 ml/min
Detection UV 250 nm

 

Description

Class of Compounds Drug, Acid
Analyzing Compounds Nicotinic Acid, Nicotinamide, Pyridine Dicarboxylic Acid, Ethyl Methyl Pyridine and Ethyl Nicotinate

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
2,5-Pyridinedicarboxylic acid
5-Ethyl-2-methylpyridine
Nicotinamide
Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)
Pyridinedicarboxylic Acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Analysis of Nicotinic Acid and Nicotinamide (Vitamin B3) on Newcrom AH Column

July 6, 2015


HPLC.cloud
View on hplc.cloud

Nicotinamide is the amide form of nicotinic acid. Nicotinic acid is in the vitamin B group and is converted to nicotinamide in vivo. Newcrom AH  was used to separate nicotinamide and nicotinic acid.  Newcrom AH separates acids using ion-exclusion, retains basic compounds by cation-exchange, and neutral compounds by reverse-phase mechanism.

Condition

Column Newcrom AH, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 50/50%
Buffer H3PO4 – 0.5%
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

Description

Class of Compounds
 Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Nicotinic Acid, Nicotinamide, Vitamin B3

 

Application Column

Newcrom AH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Niacin
Nicotinamide
Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Nicotinamide and Related Substances

May 11, 2015

A complex mixture of nicotinamide and related impurities was separated on Obelisc R mixed-mode column. Nicotinamide, methylnicotinamide, nicotinamide adenine mononucleotide, nicotinamide adenine dinucleotide, and adenosine monophosphate were baseline resolved in a 15 minute long method. This mixed-mode approach can be used for analysis of other nucleotides. Obelisc R trimodal column separates this complex mixture based on reversed-phase, cation-exchange and anion-exchange mechanisms. Retention is controlled by amount of ACN, buffer concentration and buffer pH. Additional selectivity can be gained by exploring various buffers within the same pH

Application Column

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:
1-Methylnicotinamide
Adenosine Monophosphate
Nicotinamide
Nicotinamide Adenine Dinucleotide (NAD)
Nicotinamide Adenine Mononucleotide

Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.