| CAS Number | 119-53-9 |
|---|---|
| Molecular Formula | C14H12O2 |
| Molecular Weight | 212.249 |
| InChI Key | ISAOCJYIOMOJEB-UHFFFAOYSA-N |
| LogP | 2.13 |
| Synonyms |
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Applications:
HPLC Method for Analysis of Benzoin on Primesep 200 Column
February 20, 2026
HPLC Method for Benzoin on Primesep 200 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Epibatidine
Benzoin is an organic compound with the chemical formula C14H12O2. It is primarily used as a precursor to benzil or in powder coating. You can find detailed UV spectra of Benzoin and information about its various lambda maxima by visiting the following link.
Benzoin can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
| Column | Primesep 200, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 40/60% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/m |
| Detection | UV 200 nm |
| Class of Compounds | Organic Compound |
| Analyzing Compounds | Benzoin |
Application Column
Primesep 200
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Uv-Vis Spectrum of Benzoin
February 20, 2026
Access the UV-Vis Spectrum SIELC Library

If you are looking for optimized HPLC method to analyze Benzoin check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
Reverse Mode vs Normal Mode: Separation of Aromatic Compounds
August 6, 2005

Primesep 100 separates a mixture of benzyl compounds in both normal- and reversed-phase modes with UV detection. The peak order of benzil, benzoin, bibenzylbenzoate, bibenzyl, deoxybenzoin, and 4-methyldeoxybenzoin are completely reversed with a change of mobile phase. The reversed-phase mobile phase of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) and the normal phase mobile phase of hexane and isopropanol both separate this mixture in less than 12 minutes total elution time.
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsBenzil
Benzoin
Benzylbenzoate
Bibenzyl
Deoxybenzoin



