HPLC Method for Analysis of Benzoin on Primesep 200 Column

HPLC Method for Benzoin on Primesep 200 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Epibatidine

Benzoin is an organic compound with the chemical formula C14H12O2. It is primarily used as a precursor to benzil or in powder coating. You can find detailed UV spectra of Benzoin and information about its various lambda maxima by visiting the following link.

Benzoin can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.

ColumnPrimesep 200, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 40/60%
BufferH2SO4 – 0.1%
Flow Rate1.0 ml/m
DetectionUV 200 nm
Class of Compounds
 Organic Compound
Analyzing CompoundsBenzoin

Application Column

Primesep 200

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Benzoin
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.