| CAS Number | 15972-60-8 |
|---|---|
| Molecular Formula | C14H20ClNO2 |
| Molecular Weight | 269.770 |
| InChI Key | XCSGPAVHZFQHGE-UHFFFAOYSA-N |
| LogP | 3.52 |
| Synonyms |
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Applications:
HPLC Method for Analysis of Alachlor on Primesep 100 Column
July 31, 2024
High Performance Liquid Chromatography (HPLC) Method for Analysis of Alachlor on Primesep 100 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Alachlor
Alachlor is a chloroacetanilide herbicide used to control annual grasses and broadleaf weeds primarily in corn and soybean crops. It is known for its pre-emergent activity, meaning it is applied to the soil to prevent weed germination and growth.
Uses:
- Agriculture: Primarily used in the cultivation of corn, soybeans, and other crops to manage weeds.
- Weed Control: Effective against a wide range of weeds, including annual grasses and broadleaf weeds.
Degradation:
- Biodegradation: Alachlor can be broken down by microbial activity in the soil.
- Photodegradation: Exposure to sunlight can also degrade alachlor, reducing its persistence in the environment.
Alachlor be retained and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 200 nm
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN – 55% |
| Buffer | H2SO4 -0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 nm |
| Samples | 1.0 mg/ml in MeCN – 100% |
| Injection volume | 1 µl |
| LOD* | 8 ppb (200 nm) |
| Class of Compounds | chloroacetanilide herbicides |
| Analyzing Compounds | Alachlor |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
UV-Vis Spectrum of Alachlor
July 12, 2024
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
HPLC Method for Analysis of Pesticides: Thiamphenicol, Aniline, Simazine, Alachlor, Thiabendazole, Diazinon, Trifluralin on Primesep B2 Column
July 11, 2017
| Column | Primesep B2, 3.2×100 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN – 35-85%, 6 min , 4 min hold |
| Buffer | Gradient H2SO4 – 0.05- 0.15%, 6 min, 4 min hold |
| Flow Rate | 0.6 ml/min |
| Detection | UV, 230 nm |
| Class of Compounds |
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Thiamphenicol, Aniline, Simazine, Alachlor, Thiabendazole, Diazinon, Trifluralin |
Application Column
Primesep B2
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsAniline
Diazinon
Simazine
Thiabendazole
Thiamphenicol
Trifluralin
Alachlor Separation on Obelisc R Column
August 24, 2015
| Column | Obelisc R, 2.1×150 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN – 10-70%, 15 min, 7 min hold |
| Buffer | Gradient AmAc pH 4,5- 20-50 mM, 15 min, 7 min hold |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds |
Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Alachlor |
Application Column
Obelisc R
SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Select options
HPLC Analysis of Alachlor
August 10, 2015
Condition
| Column | Obelisc R, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | AmFm pH 3.0 |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds |
Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Alachlor |
Application Column
Obelisc R
SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Select options
HPLC Method for Analysis of Pesticides: Thiamphenicol, Aniline, Simazine, Alachlor, Thiabendazole, Diazinon, Trifluralin on Primesep 100 Column
June 11, 2014
| Column | Primesep 100, 3.2×100 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN – 35-85%, 6 min , 4 min hold |
| Buffer | Gradient H2SO4 – 0.05- 0.15%, 6 min, 4 min hold |
| Flow Rate | 0.6 ml/min |
| Detection | UV, 230 nm |
| Class of Compounds |
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Thiamphenicol, Aniline, Simazine, Alachlor, Thiabendazole, Diazinon, Trifluralin |
Application Column
Primesep 100
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Alachlor
Diazinon
Simazine
Thiabendazole
Thiamphenicol
Trifluralin
Alachlor Separation on Primesep B2 Column
June 12, 2010
| Column | Primesep B2, 2.1×150 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN – 10-70%, 15 min, 7 min hold |
| Buffer | Gradient AmAc pH 4,5- 20-50 mM, 15 min, 7 min hold |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds |
Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Alachlor |
Application Column
Primesep B2
Column Diameter: 2.1 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
