HPLC Analysis of Alachlor

Alachlor is a chloroacetanilide family herbicide which inhibits parts of the gibberellin pathway. The EPA classifies alachlor as slightly toxic and set the maximum contaminant level at 2 ppb. The EU banned alachlor in 2006. Obelisc R was used to retain alachlor and separate it from impurities. Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations. Method is LC/MS compatible and can be used as a general approach for analyzing alachlor.


Column Obelisc R, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm pH 3.0
Flow Rate 1.0 ml/min
Detection UV, 250 nm


Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Alachlor
Application Analytes: