High Performance Liquid Chromatography (HPLC) Method for Analysis of Alachlor on Primesep 100 by SIELC Technologies
Alachlor is a chloroacetanilide herbicide with the chemical formula C14H20ClNO2. It is used to control annual grasses and broadleaf weeds primarily in corn and soybean crops. It is known for its pre-emergent activity, meaning it is applied to the soil to prevent weed germination and growth.
Uses:
- Agriculture: Primarily used in the cultivation of corn, soybeans, and other crops to manage weeds.
- Weed Control: Effective against a wide range of weeds, including annual grasses and broadleaf weeds.
Degradation:
- Biodegradation: Alachlor can be broken down by microbial activity in the soil.
- Photodegradation: Exposure to sunlight can also degrade alachlor, reducing its persistence in the environment.
You can find detailed UV spectra of Alachlor and information about its various lambda maxima by visiting the following link.
Alachlor be retained and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 200 nm
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 55% |
| Buffer | H2SO4 -0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 nm |
| Samples | 1.0 mg/ml in MeCN – 100% |
| Injection volume | 1 µl |
| LOD* | 8 ppb (200 nm) |
| Class of Compounds | chloroacetanilide herbicides |
| Analyzing Compounds | Alachlor |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
