HPLC Method for Analysis of Alachlor on Primesep 100 Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Alachlor on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

HPLC Method for Analysis of Alachlor on Primesep 100 Column


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Alachlor

Alachlor is a chloroacetanilide herbicide used to control annual grasses and broadleaf weeds primarily in corn and soybean crops. It is known for its pre-emergent activity, meaning it is applied to the soil to prevent weed germination and growth.

Uses:

  • Agriculture: Primarily used in the cultivation of corn, soybeans, and other crops to manage weeds.
  • Weed Control: Effective against a wide range of weeds, including annual grasses and broadleaf weeds.

Degradation:

  • Biodegradation: Alachlor can be broken down by microbial activity in the soil.
  • Photodegradation: Exposure to sunlight can also degrade alachlor, reducing its persistence in the environment.

Alachlor be retained and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 200 nm

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN – 55%
BufferH2SO4 -0.1%
Flow Rate1.0 ml/min
DetectionUV 200 nm
Samples1.0 mg/ml in MeCN – 100%
Injection volume1 µl
LOD*8 ppb (200 nm)
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
chloroacetanilide herbicides
Analyzing CompoundsAlachlor

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Alachlor
Application Detection:
UV Detection
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